pcdna1 flag atxn1 cag 85  (Addgene inc)

Journal: iScience

Article Title: Alterations in oligodendrocyte transcriptional networks reveal region-specific vulnerabilities to neurological disease

doi: 10.1016/j.isci.2023.106358

Figure Lengend Snippet:

Article Snippet: Cells were first lipofectamine transfected with 2.5 μg of either the pcDNA1 Flag ATXN1[30Q] (addgene #33236) or pcDNA1 Flag ATXN1[85Q] plasmid (addgene #33237), cultured in serum-free media with 0.05 mM PRT4165 or vehicle (0.06% DMSO) for 48 hours, and then processed for RNA-seq.

Techniques: Sample Prep, Plasmid Preparation, Software

Journal: iScience

Article Title: Alterations in oligodendrocyte transcriptional networks reveal region-specific vulnerabilities to neurological disease

doi: 10.1016/j.isci.2023.106358

Figure Lengend Snippet:

Article Snippet: Cells were first lipofectamine transfected with 2.5 μg of either the pcDNA1 Flag ATXN1[30Q] (addgene #33236) or pcDNA1 Flag ATXN1[85Q] plasmid (addgene #33237), cultured in serum-free media with 0.05 mM PRT4165 or vehicle (0.06% DMSO) for 48 hours, and then processed for RNA-seq.

Techniques: Sample Prep, Plasmid Preparation, Software